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Dr Sherif Abouelhadid

DL Tutor

United Kingdom

Before joining LSHTM, I worked on developing a chemically conjugated vaccine against Salmonella Typhi at the Egyptian Company for the Production of Vaccines, Sera, and Drugs in Cairo, Egypt. At that time, I wondered whether there was any enzyme capable of catalyzing the complex chemical reactions required to chemically link a glycan to a protein. This curiosity led me to explore the research from Wren's laboratory. I subsequently joined the Wren Lab as a research assistant, where I focused on developing glycoengineering tools, specifically targeting Streptococcus pneumoniae.

 

I thoroughly enjoyed working on this project, which inspired me to pursue a PhD in glycobiology. This time, I set myself a more challenging goal: to investigate the role of bacterial N-glycosylation. My PhD research resulted in key publications in the field. After completing my PhD in 2019, I returned to work on the translational aspects of glycobiology, primarily focusing on vaccine development and the production of glycoproteins. In 2024, I decided to allocate more time to teaching. I am now a DL tutor in IDM213. I am planning to enrich my teaching experience by joining other modules. 

Affiliations

Department of Infection Biology
Faculty of Infectious and Tropical Diseases

Centres

Centre for Epidemic Preparedness and Response
Vaccine Centre
Antimicrobial Resistance Centre

Research

My research interests are divided into two main areas: basic biology and applied science. In the field of basic biology, I focus on understanding the biological roles of N-linked glycans and exploring the functions of glycosylation systems in various organisms. I use Campylobacter jejuni, which has one of the most extensively studied glycosylation systems in bacteria, to investigate the fundamental functional roles of N-linked glycans.

 

In parallel with my basic biology research, I also work on biotechnological applications derived from my findings, particularly in utilizing bacterial oligosaccharyltransferases and/or glycosyltransferases to develop conjugate vaccines and potentially humanized glycoproteins.

 

 My curiosity drives me to explore how orchestrated biological processes work. Therefore, in addition to my primary research, I am eager to learn more about how biological systems function and how we can harness the molecular machinery to develop biomimetic or bio-inspired tools.

Research Area
Bacteria
Vaccines
Bacteriology
Biochemistry
Microbiology
Molecular biology
Vaccinology
Disease and Health Conditions
Infectious diseases

Selected Publications

Quantitative Analyses Reveal Novel Roles for N-Glycosylation in a Major Enteric Bacterial Pathogen.
ABOUELHADID, S; North, SJ; Hitchen, P; Vohra, P; Chintoan-Uta, C; Stevens, M; Dell, A; CUCCUI, J; WREN, BW;
2019
mBio
Recombinant production platform for Group A Streptococcus glycoconjugate vaccines.
Ajay Castro, S; PASSMORE, IJ; Ndeh, D; Shaw, HA; Ruda, A; Burns, K; Thomson, S; Nagar, R; Alagesan, K; Reglinski, M; Lucas, K; ABOUELHADID, S; Schwarz-Linek, U; Mawas, F; Widmalm, G; WREN, BW; Dorfmueller, HC;
2025
NPJ vaccines
Investigation into the efficiency of diverse N-linking oligosaccharyltransferases for glycoengineering using a standardised cell-free assay.
Lehri, B; ATKINS, E; SCOTT, TA; ABOUELHADID, S; WREN, BW; CUCCUI, J;
2024
Microbial biotechnology
A platform for the recombinant production of Group A Streptococcus glycoconjugate vaccines
Castro, SA; PASSMORE, IJ; Ndeh, D; Shaw, HA; Ruda, A; Burns, K; Thomson, S; Nagar, R; Alagesan, K; Lucas, K; ABOUELHADID, S; Reglinski, M; Schwarz-Linek, U; Mawas, F; Widmalm, G; WREN, BW; Dorfmueller, HC;
2024
Cold Spring Harbor Laboratory
Development of a novel glycoengineering platform for the rapid production of conjugate vaccines.
ABOUELHADID, S; ATKINS, ER; Kay, EJ; PASSMORE, IJ; North, SJ; Lehri, B; Hitchen, P; Bakke, E; Rahman, M; Bossé, JT; Li, Y; TERRA, VS; Langford, PR; Dell, A; WREN, BW; CUCCUI, J;
2023
Microbial cell factories
A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli.
PASSMORE, IJ; Faulds-Pain, A; ABOUELHADID, S; HARRISON, MA; HALL, CL; Hitchen, P; Dell, A; Heap, JT; WREN, BW;
2023
Glycobiology
Enhanced Zika virus-like particle development using Baculovirus spp. constructs.
Malogolovkin, A; Davies, A; ABOUELHADID, S; Kerviel, A; ROY, P; Falconar, AK;
2022
Journal of medical virology
Development of a novel glycoengineering platform for the rapid production of conjugate vaccines
ABOUELHADID, S; Atkins, E; Kay, E; Passmore, I; North, S; Lehri, B; Hitchen, P; Bakke, E; Rahman, M; Bosse, J; Li, Y; Terra, V; Langford, P; Dell, A; Wren, B; Cuccui, J;
2021
bioRxiv
Multivalent poultry vaccine development using Protein Glycan Coupling Technology.
MAURI, M; Sannasiddappa, TH; Vohra, P; Corona-Torres, R; Smith, AA; Chintoan-Uta, C; Bremner, A; TERRA, VS; ABOUELHADID, S; Stevens, MP; Grant, AJ; CUCCUI, J; WREN, BW; Glycoengineering of Veterinary Vaccines Consortium,;
2021
Microbial cell factories
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