Streamlining the production of recombinant proteins for structural proteomics of human and pathogen proteins

Structural proteomics is the comprehensive analysis of protein structures in the context of whole proteomes. The field originally emerged in response to the acceleration of genomic sequencing and the de-coding of the human genome and that of many pathogens. Over the last ten years, structural proteomics has become a mature discipline focused on specific structural biological problems or the proteomes of specific cells or environments. As one of the first Structural Proteomics Facilities in Europe, we have developed a range of technologies incorporating robotic systems to enable the high throughput cloning, expression and purification of recombinant proteins. Our experience in developing a high throughput pipeline for protein production will be reviewed with reference to recent projects of pathogen proteins.