Rapid, iterative and scalable genome editing in P. knowlesi
Rapid, iterative and scalable genome editing in P. knowlesi : from basic science to vaccine design”
CRISPR Cas9 genome editing has revolutionised many fields of research, including through its recent application to P. falciparum. The system has been adapted for use in the zoonotic malaria parasite P. knowlesi and adopted innovative approaches for construct generation which enables fast, markerless and scalable modification of the genome.
This has allowed investigation into parasite biology in new and ambitious ways including helping us develop tools to understand parasite invasion and also using P. knowlesi as a surrogate to test potential vaccines against P. vivax - an important malaria pathogen which cannot be maintained in culture.