Close

Wolfson Cell Biology Facility

Part of the Faculty of Infectious and Tropical Diseases, and based at our Keppel Street site. The WCBF has been established to enhance the quality of research from staff at the School by providing access to, and specialised training for a range of equipment in pursuit of excellence within laboratory-based research. The equipment within this facility are open access to both staff and students of the School as well as external scientists. Please contact us to discuss your requirements.

Our staff

Theresa Ward Academic in Charge of Imaging Facility
Liz McCarthy Imaging, Biacore and Real Time PCR
Rachel Gregory Imaging, Biacore and Real Time PCR
Liz King Flow cytometry and Luminex

Our equipment

Confocal Microscopy

We have an inverted Zeiss LSM510 confocal microscope with four lasers: laser diode, 405nm; Argon 458, 488 and 514nm; HeNe1, 543nm; and HeNe2, 633nm.

The LSM510 has a fully motorized stage allowing for z stack acquisition and tile scanning.  Used in combination with the CO2 and temperature environmental controls we can also image multiple locations over time.  This can be stabilised using the autofocus functionality.

FRAP (Fluorescence Recovery After Photobleaching) and FRET (Fluorescence Resonance Energy Transfer) experiments are also possible with this system.

The microscope is also equipped with a Femtojet microinjection unit allowing for the introduction of DNA, RNA or drugs into individual cells.

Widefield Microscopy

Nikon Ti E

We have an inverted Nikon Ti Eclipse microscope with OKO-lab environmental controls and fully motorized stage allowing for live cell imaging, tile scanning and the imaging of multiple locations.  The piezo stage provides high speed z-axis control, perfect for rapid z-stack acquisition. The system comes equipped with the Perfect Focus System (PFS) for stabilisation of live cell imaging.

A variety of stage inserts and objectives (including extra long working distance, ELWD, lenses) enables samples in a variety of formats including standard slides, dishes and multi-well plates to be imaged.

Histology

We have an upright manual Leica microscope with Retiga colour camera ideal for histology imaging.  Volocity software is utilised for image acquisition and available for image processing and quantification.

Laser Capture Microdissection

Laser Capture Microdissection (LCM) is a technique whereby a population of pure cells can be isolated from a heterogenous tissue sample.

We have an Arcturus Pixcell IIe Laser Capture Microdissection System. This system uses an infra-red (IR) laser to capture cells of interest onto a transparent film placed above the sample. The microscope is equipped with x5, x10, x20, and x40 lenses, with fluorescence capabilities. LCM can be used on a variety of sample preparations such as blood smears, cell cultures and tissue sections.

Image Analysis

In addition to the open source softwares: Image J/FIJI and Cell Profiler.  Staff and students of the School can use Volocity from PerkinElmer, or NIS-Elements from Nikon.  Guidance and/or consultations for your specific requirements are available.

Flow Cytometry

Biacore

7500 Fast Real Time PCR machine

The Applied Biosystems 7500 Fast RT PCR system provides high quality PCR quantification in minimal time.  Using a 96-well format, a range of dyes can be used including: Cy3, Cy5, FAM, JOE, NED, SYBR Green, TAMRA, Texas Red and VIC.