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Dr Jon Cuccui

BSc MSc PhD

Assistant Professor
Molecular Bacteriology

Room
Room 204b

LSHTM
Keppel Street
London
WC1E 7HT
United Kingdom

Tel.
+44 (0) 20 7927 2813

Fax.
+44 (0) 20 7637 4314

I joined the LSHTM in 2002 as a research assistant and registered as a Ph.D. student  in Professor Brendan Wren's laboratory carrying out signature tagged mutagenesis on Burkholderia pseudomallei. My first Post Doc involved developing and utilising a novel glycoengineering method termed protein glycan coupling technology using Francisella tularensis and B. pseudomallei as the test organisms. My current fellowship revolves around defining the limitations of bacterial glycosylation systems and how to overcome these for potential exploitation.

 My interests are not only on the application and development of novel vaccine strategies and technologies but also on the biological reasons why bacteria carry varied glycosylation systems. 

 

Affiliations

Faculty of Infectious and Tropical Diseases
Department of Pathogen Molecular Biology

Centres

Vaccine Centre

Teaching

 

Ph.D. supervisor and Medical Microbiology M.Sc. lecturer, tutor, project supervisor, examiner and exam board member.

Research

My aim is to enhance our understanding of bacterial glycosylation systems and to also identify areas that may be exploited for glycoengineering purposes. I was part of a team who developed a first generation glycoconjugate vaccine against Francisella tularensis using the O-antigen repeat unit from the organism as the glycan component. I was also involved in the completion of a project attempting to characterise the capsular polysaccharide of Burkholderia pseudomallei. I maintain an interest on the work being carried out within the Wren laboratory to further understand selected mutants identified during my PhD entitled 'The development of a refined signature tagged mutagenesis strategy for the identification of novel virulence determinants and vaccine candidates in Burkholderia pseudomallei.' Recently, I have also been involved in a Transposon Directed Insertion Site sequencing project that aimed to enhance our understanding of B. pseudomallei.  

Currently I am funded by a Wellcome Trust/LSHTM fellowship to identify and overcome glycoengineering bottlenecks in order to expand the capabilties of a technique termed protein glycan coupling technology. I am also studying the N-linked glycosylation system in the porcine pathogen A. pleuropneumoniae and attempting to unravel its biological function and potential application. More recently I was a successful co-applicant on a BBSRC LoLa grant aimed at developing novel vaccines for the Poultry and ruminant market.

Research Area
Bacteria
Vaccines
Discipline
Genetic epidemiology
Bacteriology
Genetics
Microbiology
Molecular biology
Disease and Health Conditions
Infectious disease

Selected Publications

The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering.
Cuccui, J. ; Terra, V.S. ; Bossé, J.T. ; Naegeli, A. ; Abouelhadid, S. ; Li, Y. ; Lin, C.W. ; Vohra, P. ; Tucker, A.W. ; Rycroft, A.N. ; Maskell, D.J. ; Aebi, M. ; Langford, P.R. ; Wren, B.W. ; BRaDP1T Consortium, . ;
Open Biol
Functional analysis of N-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria.
Mills, D.C.; Jervis, A.J.; Abouelhadid, S.; Yates, L.E.; Cuccui, J.; Linton, D.; Wren, B.W.;
Glycobiology
Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli.
Kay, E.J. ; Yates, L.E. ; Terra, V.S. ; Cuccui, J. ; Wren, B.W. ;
Open Biol
Genome-Wide Saturation Mutagenesis of Burkholderia pseudomallei K96243 Predicts Essential Genes and Novel Targets for Antimicrobial Development.
Moule, M.G. ; Hemsley, C.M. ; Seet, Q. ; Guerra-Assunção, J.A. ; Lim, J. ; Sarkar-Tyson, M. ; Clark, T.G. ; Tan, P.B. ; Titball, R.W. ; Cuccui, J. ; Wren, B.W. ;
MBio
Growth Inhibition of Pathogenic Bacteria by Sulfonylurea Herbicides.
Kreisberg, J.F.; Ong, N.T.; Krishna, A.; Joseph, T.L.; Wang, J.; Ong, C.; Ooi, H.A.; Sung, J.C.; Siew, C.C.; Chang, G.C.; Biot, F.; Cuccui, J.; Wren, B.W.; Chan, J.; Sivalingam, S.P.; Zhang, L.H.; Verma, C.; Tan, P.;
Antimicrob Agents Chemother
Exploitation of bacterial N-linked glycosylation to develop a novel recombinant glycoconjugate vaccine against Francisella tularensis.
Cuccui, J. ; Thomas, R.M. ; Moule, M.G. ; D'Elia, R.V. ; Laws, T.R. ; Mills, D.C. ; Williamson, D. ; Atkins, T.P. ; Prior, J.L. ; Wren, B.W. ;
Open Biol
Bacteria like sharing their sweets.
Cuccui, J. ; Wren, B.W. ;
Mol Microbiol
Characterisation of the Burkholderia pseudomallei K96243 CPS I coding region.
Cuccui, J.; Milne, T.S.; Harmer, N.; George, A.J.; Harding, S.V.; Dean, R.E.; Scott, A.E.; Sarkar-Tyson, M.; Wren, B.W.; Titball, R.W.; Prior, J.L.;
Infect Immun
See all Dr Jon Cuccui's Publications