Dr Jon Cuccui


Assistant Professor
Molecular Bacteriology

Room 204b

Keppel Street
United Kingdom

+44 (0) 20 7927 2813

+44 (0) 20 7637 4314

I joined the LSHTM in 2002 as a research assistant and registered as a Ph.D. student  in Professor Brendan Wren's laboratory carrying out signature tagged mutagenesis on Burkholderia pseudomallei. My first Post Doc involved developing and utilising a novel glycoengineering method termed protein glycan coupling technology using Francisella tularensis and B. pseudomallei as the test organisms. My current fellowship revolves around defining the limitations of bacterial glycosylation systems and how to overcome these for potential exploitation.

 My interests are not only on the application and development of novel vaccine strategies and technologies but also on the biological reasons why bacteria carry varied glycosylation systems. 



Faculty of Infectious and Tropical Diseases
Department of Pathogen Molecular Biology


Vaccine Centre



Ph.D. supervisor and Medical Microbiology M.Sc. lecturer, tutor, project supervisor, examiner and exam board member.


My aim is to enhance our understanding of bacterial glycosylation systems and to also identify areas that may be exploited for glycoengineering purposes. I was part of a team who developed a first generation glycoconjugate vaccine against Francisella tularensis using the O-antigen repeat unit from the organism as the glycan component. I was also involved in the completion of a project attempting to characterise the capsular polysaccharide of Burkholderia pseudomallei. I maintain an interest on the work being carried out within the Wren laboratory to further understand selected mutants identified during my PhD entitled 'The development of a refined signature tagged mutagenesis strategy for the identification of novel virulence determinants and vaccine candidates in Burkholderia pseudomallei.' Recently, I have also been involved in a Transposon Directed Insertion Site sequencing project that aimed to enhance our understanding of B. pseudomallei.  

Currently I am funded by a BBSRC RSE Enterprise Fellowship to learn about the process of forming a spin out from experienced business leaders. I have a partiular interest in the translational aspect of glycobiology. I am also a co-applicant on a BBSRC LoLa grant aiming to develop novel vaccines for the Poultry and ruminant markets.

Research Area
Genetic epidemiology
Molecular biology
Disease and Health Conditions
Infectious disease

Selected Publications

Structure-activity relationships in a new class of non-substrate-like covalent inhibitors of the bacterial glycosyltransferase LgtC.
Xu, Y. ; Cuccui, J. ; Denman, C. ; Maharjan, T. ; Wren, B.W. ; Wagner, G.K. ;
Bioorg Med Chem
The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering.
Cuccui, J. ; Terra, V.S. ; Bossé, J.T. ; Naegeli, A. ; Abouelhadid, S. ; Li, Y. ; Lin, C.W. ; Vohra, P. ; Tucker, A.W. ; Rycroft, A.N. ; Maskell, D.J. ; Aebi, M. ; Langford, P.R. ; Wren, B.W. ; BRaDP1T Consortium, . ;
Open Biol
Functional analysis of N-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria.
Mills, D.C.; Jervis, A.J.; Abouelhadid, S.; Yates, L.E.; Cuccui, J.; Linton, D.; Wren, B.W.;
Genome-Wide Saturation Mutagenesis of Burkholderia pseudomallei K96243 Predicts Essential Genes and Novel Targets for Antimicrobial Development.
Moule, M.G. ; Hemsley, C.M. ; Seet, Q. ; Guerra-Assunção, J.A. ; Lim, J. ; Sarkar-Tyson, M. ; Clark, T.G. ; Tan, P.B. ; Titball, R.W. ; Cuccui, J. ; Wren, B.W. ;
Exploitation of bacterial N-linked glycosylation to develop a novel recombinant glycoconjugate vaccine against Francisella tularensis.
Cuccui, J. ; Thomas, R.M. ; Moule, M.G. ; D'Elia, R.V. ; Laws, T.R. ; Mills, D.C. ; Williamson, D. ; Atkins, T.P. ; Prior, J.L. ; Wren, B.W. ;
Open Biol
Bacteria like sharing their sweets.
Cuccui, J. ; Wren, B.W. ;
Mol Microbiol
Characterisation of the Burkholderia pseudomallei K96243 CPS I coding region.
Cuccui, J.; Milne, T.S.; Harmer, N.; George, A.J.; Harding, S.V.; Dean, R.E.; Scott, A.E.; Sarkar-Tyson, M.; Wren, B.W.; Titball, R.W.; Prior, J.L.;
Infect Immun
See all Dr Jon Cuccui's Publications