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Dr Khalid Beshir

BSc MSc PhD

Assistant Professor

Room
Room 390

LSHTM
Keppel Street
London
WC1E 7HT
United Kingdom

Khalid has background in Biology, Medical Parasitology and Biomedical Science (microbiology). Khalid completed his PhD under the supervision of Prof Colin Sutherland.

Dr Beshir's research work focuses on understanding how malaria parasites evade drug attack (become resistant) and escape detection by rapid diagnostic tests (RDTs)and become invisible. To achieve this, we continuously work on development of new and fit-for-purpose molecular tools to detect and monitor these new malaria parasite population. Dr Beshir has recently started working on the development of affordable COVID-19 testing assays that can be used in Low and Middle Income Countries.

Dr Beshir previously worked on EDCTP-funded WANECAM project on Clinical trial of four Artemisinin Combination Therapies (ACT) in west Africa (with Prof Sutherland and Prof Abdoulaye Djimde). Dr Beshir also worked on molecular evaluation of the efficacy of ACTs as well as Seasonal Malaria Chemoprevention (SMC) therapies in West Africa (with Prof Sutherland and Prof Paul Milligan). In both projects, Dr Dr Beshir worked on development of new molecular tools to monitor the emergence of drug resistance parasites to both ACTs and SMCs.

Dr Beshir is also involved in teaching both within LSHTM and externally, and supervision of MSc and PhD students. Dr Beshir is also a member of LSHTM ethics commitee and faculty member of Equality, Diversity and Inclusion.

Affiliations

Department of Infection Biology
Faculty of Infectious and Tropical Diseases

Centres

Malaria Centre
Antimicrobial Resistance Centre (AMR)
The Applied Genomics Centre

Teaching

Dr Beshir is a tutor on Medical Parasitology course and Malaria DL module and is involved in the teaching of Miscroscopic Diagnosis of Malaria Parasites and Molecular Biology. Dr Beshir is a member of joint committee for Medical Parasitology and Medical Entomology for Disease Control courses.

Research

Dr Beshir's project involves understadning how malaria parasites evade detection by RDTs and become invisible; the impact of the change on the RDT perfromance; the possible fitness-cost to the malaria parasites due to the change in their genetic make-up to achieve the invisibility and what effect this has on disease outcome and severity. Dr Beshir's research focuses in three areas:

1. Understanding how malaria parasites escape detection. To achieve this, we developed a molecular tool called multiplex qPCR to identify the genes that help the pathogen become invisible during diagnosis by RDT. We have shown that the pathogen deletes two key genes (pfhrp2 and pfhrp3), its antigens commonly recognised by RDT.  Our work has previously identified these striain of malaria pathgens in Eritrea, Ethiopia, Kenya, Somalia, South Sudan, Sudan, Tanzania and Uganda.

2. Understading the consequence of gene deletion to the fitness of the malaria pathogen. We investigate whether the deletion of the genes cause the parasite to be less fit by measuring its ability to grow and develop in the labouratory.

3. Investigating whether malaria pathogens with the deletion of the two key genes have any impact on the outcome and severity of disease. We are currently working on samples from mild and severe malaria cases to see if the two groups are infected with different malaria parasite populations.

In Dr Sutherlands Lab, Dr Beshir is also involved in :

1. Development of novel molecular tools to understand why some malaria pathogens evade drug attack (become resistant). Dr Beshir, in collaboration with Dr Sutherland, has been continuously working to assess the efficacy of drugs by developing novel and fit-for-purpose molecular tools. The molecular tools that we previously developed have been used in drug resitance studies in Africa.

2. Investigation and identification of potential molecular markers for ACT resistance  in Plasmodium falciparum. Dr Beshir is particularily interested in genes involved in causing the malaria drugs to fail in killing the malaria parastes. Genes such as pfcrt, pfmdr1, pfK13, Pfcoronin and Pfaptmu are our current targets to investigate for mutations.

Research Area
Clinical trials
Diagnostics
Drug resistance
Parasites
Chemotherapy
Discipline
Genetic epidemiology
Genomics
Molecular epidemiology
Molecular biology
Parasitology
Disease and Health Conditions
Infectious disease
Malaria
Country
Afghanistan
Burkina Faso
Eritrea
Ethiopia
Ghana
Guinea
Indonesia
Kenya
Mali
Niger
Nigeria
Sudan
Senegal
Somalia
South Sudan
Chad
Tanzania
Uganda
Region
South Asia
Sub-Saharan Africa (all income levels)

Selected Publications

Persistent Submicroscopic Plasmodium falciparum Parasitemia 72 Hours after Treatment with Artemether-Lumefantrine Predicts 42-Day Treatment Failure in Mali and Burkina Faso.
Beshir KB; Diallo N; Somé FA; Sombie S; Zongo I; Fofana B; Traore A; Dama S; Bamadio A; Traore OB
2021
Antimicrobial agents and chemotherapy
Antimalarial drug resistance markers in human immunodeficiency virus (HIV)-positive and HIV-negative adults with asymptomatic malaria infections in Port Harcourt, Nigeria.
Chijioke-Nwauche I; Oguike MC; Nwauche CA; Beshir KB; Sutherland CJ
2021
Transactions of the Royal Society of Tropical Medicine and Hygiene
Failure of rapid diagnostic tests in Plasmodium falciparum malaria cases among travelers to the UK and Ireland: Identification and characterisation of the parasites.
Nolder D; Stewart L; Tucker J; Ibrahim A; Gray A; Corrah T; Gallagher C; John L; O'Brien E; Aggarwal D
2021
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
Emergence of Undetectable Malaria Parasites: A Threat under the Radar amid the COVID-19 Pandemic?
Beshir KB; Grignard L; Hajissa K; Mohammed A; Nurhussein AM; Ishengoma DS; Lubis IND; Drakeley CJ; Sutherland CJ
2020
The American journal of tropical medicine and hygiene
Plasmodium falciparum isolate with histidine-rich protein 2 gene deletion from Nyala City, Western Sudan.
Boush MA; Djibrine MA; Mussa A; Talib M; Maki A; Mohammed A; Beshir KB; Mohamed Z; Hajissa K
2020
SCIENTIFIC REPORTS
A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections.
Grignard L; Nolder D; Sepúlveda N; Berhane A; Mihreteab S; Kaaya R; Phelan J; Moser K; van Schalkwyk DA; Campino S
2020
EBioMedicine
Plasmodium falciparum Isolates Carrying pfk13 Polymorphisms Harbor the SVMNT Allele of pfcrt in Northwestern Indonesia.
Lubis IND; Wijaya H; Lubis M; Lubis CP; Beshir KB; Sutherland CJ
2020
Antimicrobial agents and chemotherapy
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