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Dr Khalid Beshir

BSc MSc PhD

Assistant Professor

Room
Room 390

LSHTM
Keppel Street
London
WC1E 7HT
United Kingdom

Khalid joined the School to work on EDCTP-funded WANECAM project - Clinical trial of four Artemisinin Combination Therapies (ACT) carried out in 5 sites within three West African countries: Mali, Burkina Faso and Guinea Conakry. Khalid's work focuses on development of new molecular tools to monitor the emergence of drug resistance parasites in the era of ACT. Khalid is currently working on molecular evaluation of the efficacy of ACTs as well as Seasonal Malaria Chemoprevention (SMC) therapies in West Africa. 

Dr Beshir's other project involve investigation of deletion of Plasmodium falciparum histidine-rich proteins (Pfhrp2 and pfhrp3) and the impact on RDT perfomance, parasite genome profile and disease outcome. We have recently reported that malaria parasites with gene deletions that affect a key target of rapid diagnostic tests (RDTs) are circulating in East Africa. We identified P. falciparum parasites from eastern and western Kenya that have deletions of the genes, which code for histidine-rich proteins 2 (HRP2) and 3 (HRP3). We are currently analyzing parasite samples from Ghana, Tanzania and Uganda, to investigate whether pfhrp2 and pfhrp3 deletions are present, and to integrate molecular data with microscopy and RDT results to assess any effect on RDT performance in those areas.

Affiliations

Department of Immunology and Infection
Faculty of Infectious and Tropical Diseases

Centres

Malaria Centre
Antimicrobial Resistance Centre

Teaching

Khalid is a tutor on Medical Parasitology course and Malaria DL module and is involved in the teaching of Miscroscopic Diagnosis of Malaria Parasites and Molecular Biology. Khalid is a member of joint committee for Medical Parasitology and Medical Entomology for Disease Control courses.

Research

 In the Sutherland lab, Khalid's research focuses in three areas:

1.Development of novel molecular tools to monitor the emergence of artemisinin resistant parasites in the era of ACT. Khalid, under the supervision of Dr Sutherland, has recently developed a realtime PCR tool to measure parasite clearance after ACT treatment. Currently, the assay is bieng used on DNA derived from filter-paper bloodspots of pateints from different malaria endemic countries.

2. Investigation and identification of potential molecular markers for ACT resistance  in Plasmodium falciparum. Khalid is particularily interested in two genes, which code for Plasmodium falciparum transporter proteins; namely Plasmodium falciparum chloroquine resistance transporter (pfcrt) and Plasmodium flaciparum multidrug resistance 1 (pfmdr1).

3. Tracking Plasmodium falciparum with histidine-rich protein (pfhrp2 and pfhrp3) deletions in East Africa: unravelling the genomic profile of the parasites and the impact on clinical outcome 

Research Area
Clinical trials
Drug resistance
Parasites
Chemotherapy
Discipline
Genetic epidemiology
Molecular epidemiology
Molecular biology
Parasitology
Disease and Health Conditions
Infectious disease
Malaria
Country
Afghanistan
Burkina Faso
Eritrea
Ethiopia
Ghana
Guinea
Indonesia
Kenya
Mali
Niger
Nigeria
Sudan
Chad
Tanzania
Uganda
Region
South Asia
Sub-Saharan Africa (all income levels)

Selected Publications

Plasmodium falciparum parasites with histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in two endemic regions of Kenya.
Beshir, K.B. ; Sepúlveda, N. ; Bharmal, J. ; Robinson, A. ; Mwanguzi, J. ; Busula, A.O. ; de Boer, J.G. ; Sutherland, C. ; Cunningham, J. ; Hopkins, H. ;
2017
Sci Rep
Lack of K13 mutations in Plasmodium falciparum persisting after artemisinin combination therapy treatment of Kenyan children.
Muwanguzi, J. ; Henriques, G. ; Sawa, P. ; Bousema, T. ; Sutherland, C.J. ; Beshir, K.B. ;
2016
Malar J
Directional selection at the pfmdr1, pfcrt, pfubp1 and pfap2mu loci of Plasmodium falciparum in Kenyan children treated with ACT.
Henriques, G.; Hallett, R.L.; Beshir, K.B.; Gadalla, N.B.; Johnson, R.E.; Burrow, R.; van Schalkwyk, D.A.; Sawa, P.; Omar, S.A.; Clark, T.G.; Bousema, T.; Sutherland, C.J.;
2014
J Infect Dis
Residual Plasmodium falciparum parasitemia in Kenyan children after artemisinin-combination therapy is associated with increased transmission to mosquitoes and parasite recurrence.
Beshir, K.B.; Sutherland, C.J.; Sawa, P.; Drakeley, C.J.; Okell, L.; Mweresa, C.K.; Omar, S.A.; Shekalaghe, S.A.; Kaur, H.; Ndaro, A.; Chilongola, J.; Schallig, H.D.; Sauerwein, R.W.; Hallett, R.L.; Bousema, T.;
2013
J Infect Dis
Culture-adapted Plasmodium falciparum isolates from UK travellers: in vitro drug sensitivity, clonality and drug resistance markers.
van Schalkwyk, D.A. ; Burrow, R. ; Henriques, G. ; Gadalla, N.B. ; Beshir, K.B. ; Hasford, C. ; Wright, S.G. ; Ding, X.C. ; Chiodini, P.L. ; Sutherland, C.J. ;
2013
Malar J
Amodiaquine resistance in Plasmodium falciparum malaria is associated with the pfcrt 72-76 SVMNT allele in Afghanistan.
Beshir, K.; Sutherland, C.J.; Merinopoulos, I.; Durrani, N.; Leslie, T.; Rowland, M.; Hallett, R.L.;
2010
Antimicrob Agents Chemother
Measuring the efficacy of anti-malarial drugs in vivo: quantitative PCR measurement of parasite clearance.
Beshir, K.B.; Hallett, R.L.; Eziefula, A.C.; Bailey, R.; Watson, J.; Wright, S.G.; Chiodini, P.L.; Polley, S.D.; Sutherland, C.J.;
2010
Malar J
See all Dr Khalid Beshir's Publications