 Genetic
and molecular characterisation of the gastric pathogen Helicobacter
pylori
- Principal investigator: Brendan
Wren
- Co-Investigator: Nick Dorrell, Baraa Hussein
- Funder: MRC
- Objectives:
The objectives of the project are to determine the genetic basis
by which H. pylori causes peptic gastric disease. Specific
aims are to:
characterise phospholipase and hemolysin virulence determinants
study key genetic regulatory pathways in relation to stress adaptation
using transcriptome analysis
ascertain the importance of biofilm formation ex vivo
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Dorrell, N., Martino, M.C., Stabler, R.A., Ward, S.J., Zhang, Z.W.,
McColm, A.A., Farthing, M.J. & Wren, B.W. (1999). Characterization
of Helicobacter pylori PldA, a phospholipase with a role
in colonization of the gastric mucosa. Gastroenterology
117: 1098-104
-
Foynes, S., Dorrell, N., Ward, S.J., Zhang, Z.W., McColm, A.A.,
Farthing, M.J. & Wren, B.W. (1999). Functional
analysis of the roles of FliQ and FlhB in flagellar expression in
Helicobacter pylori. FEMS Microbiol Lett 174:
33-9
-
Allan, E., Foynes, S., Dorrell, N., Wren, B.W. & Dorrell, N.
(1999). Genetic characterisation of the gastric pathogen Helicobacter
pylori. In: Methods in Microbiology: Genetic methods for diverse
prokaryotes. M.C.M. Smith & R.E. Sockett (eds). London, Academic
Press: pp329-348
-
Allan, E., Dorrell, N., Foynes, S., Anyim, M. & Wren, B.W.
(2000). Mutational
analysis of genes encoding the early flagellar components of Helicobacter
pylori: evidence for transcriptional regulation of flagellin
A biosynthesis. Journal of Bacteriology 182: 5274-7
-
Foynes, S., Dorrell, N., Ward, S.J., Stabler, R.A., McColm, A.A.,
Rycroft, A.N. & Wren, B.W. (2000). Helicobacter
pylori possesses two CheY response regulators and a histidine
kinase sensor, CheA, which are essential for chemotaxis and colonization
of the gastric mucosa. Infect Immun 68: 2016-23
-
Hatzifoti, C., Wren, B.W. & Morrow, W.J. (2000). Helicobacter
pylori vaccine strategies--triggering a gut reaction. Immunol
Today 21: 615-9
-
Martino, M.C., Stabler, R.A., Zhang, Z.W., Farthing, M.J., Wren,
B.W. & Dorrell, N. (2001). Helicobacter
pylori pore-forming cytolysin orthologue TlyA possesses in vitro
hemolytic activity and has a role in colonization of the gastric
mucosa. Infect Immun 69: 1697-703
-
Allan, E., Clayton, C.L., McLaren, A., Wallace, D.M. & Wren,
B.W. (2001). Characterization
of the low-pH responses of Helicobacter pylori using genomic
DNA arrays. Microbiology 147: 2285-92
-
Zhang, Z.W., Dorrell, N., Wren, B.W. & Farthingt, M.J. (2002).
Helicobacter
pylori adherence to gastric epithelial cells: a role for non-adhesin
virulence genes. J Med Microbiol 51: 495-502
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Hatzifoti C., Bajaj-Elliott M., Dorrell N., Anyim M., Prentice
M.B., Nye K.E., Wren B.W. & Morrow W.J. (2004) A
plasmid immunization construct encoding urease B of Helicobacter
pylori induces an antigen-specific antibody response and upregulates
the expression of beta-defensins and IL-10 in the stomachs of immunized
mice. Vaccine 22(20):2651-2659
-
Hatzifoti C, Roussel Y, Harris A, Wren BW, Morrow WJW, Bajaj-Elliott
M (2006) Mucusal
immunisation with a urease B DNA vaccine induces innate and cellular
immune responses against Helicobacter pylori. Helicobacter
11:113-122
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