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Polly Roy MSc PhD FMedSci

Polly Roy
Professor of Virology
Room 363b, Keppel Street, London WC1E 7HT, United Kingdom
Tel: +44 (0)20 7927 2324
Fax: +44 (0)20 7927 2839

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Affiliated to: PMBD.

Disciplines: Biochemistry, Cell biology, Molecular biology.

Research areas: Bluetongue virus, HIV/AIDS, Infectious disease, Vaccines, Viruses.

Other keywords: AHSV, SARS, Rift Valley Fever, Influenza, Rotavirus, 3D Structure.


Background

Professor Polly Roy’s salient contribution has been the first complete molecular understanding of a distinct group of viruses, orbiviruses, of serious health and economic impact. This understanding, gained through a combination of virology, molecular and structural studies, has been instrumental in paving the way to improved diagnostics, vaccines and therapy. Roy used several groundbreaking techniques and multi-disciplinary approaches to provide a detailed understanding of Bluetongue virus, a major orbivirus pathogen and model for human rotavirus. Her studies have examined each aspect of the virus, from individual virus proteins to assembly of the complete virus particle and its engagement, at various levels, with the host cell. Her contribution to virology, in particular to virus structure and assembly, has been recognised by her peers worldwide. Indeed BTV, a complex double-stranded RNA virus, is now one of the most well understood viruses and Roy’s name is synonymous with it. Roy was first to demonstrate that simultaneous expression of several recombinant viral proteins leads to the assembly of virus-like particles (VLPs). This fundamental observation enabled a dissection of BTV structure and assembly pathways but has also been applied since to many other viruses including for successful vaccine development for Human Papilloma virus, Influenza and SARS. Recently Roy pioneered the first reverse genetics system for BTV (the synthesis of infectious virus solely from synthetic genes), a major achievement long sought by virologists worldwide for this class of viruses. Such directed virus genetic manipulation opens a new window of opportunity to understand how the virus invades hosts to cause disease and will benefit the development of new therapies in the longer term.  

Teaching

At LSHTM, Professor Roy is Chair of the Virology exam board. She also co-organises the M.Sc Study Units in 'Medical Microbiology' and `Molecular Virology' course, lectures in these courses and invites appropriate lecturers from other universities and institutes to teach them. She has supervised M.Sc research project students, encouraged these students and helped them to pursue further education. She is a member of the school Senior Management Team and a member of the Court of Governors at LSHTM. She is also a co-ordinator of several EU projects, and is currently carries out research with support from the EU, BBSRC and NIH.

Other International courses:

Virus and Immunity, Ecole Normale Supérieure Univ. of Lyon, France; Molecular Cell Biology, Philipps-Universität Univ. of Marburg, Germany; Nanotechnology, Univ. of Sassari, Italy; Viral Vaccines, University of Teramo, Italy; Molecular Virology, India; Virus Diagnostics, India; Molecular Virology, China; Medical Microbiology, Algeria; Microbiology, BHM Southern College, USA.

Laboratory Research Trainees:

Present and past post-doctoral research associates (UK and USA): Total 86

Present and past Ph.D. students: Total 37

Undergraduate research students: Total 24

Research

My latest research has continued to centre on basic understanding of virus infection using a multi-disciplinary approach. Current work is based on a molecular dissection of an RNA virus, Bluetongue virus, as a model system for a number of similar viruses that are pathogenic to man and animals. Our studies to date have contributed in understanding of each stage of viral life cycles including virus entry, disassembly, viral encoded enzymatic functions necessary to generate genomic RNA, assembly mechanism of newly synthesised viral components, host-virus interaction and the mechanism of virus egress.

The most recent ground breaking research development has been the recovery of infectious BTV wholly from synthetic RNA and the establishment of a helper virus-independent reverse genetics system for BTV. The ability to recover specific mutations in the genome of BTV for the first time not only provides a novel tool for the molecular dissection of BTV and related orbiviruses, but also the opportunity to develop specifically attenuated vaccines to these viruses.

The data that we have generated through our research has allowed us a number of publications in top journals including in Proceedings of the National Academy of Sciences of the United States of America (PNAS), Nature, Journal of Biological Chemistry and Federation of European Biochemical Societies letters and Journals of Virology.

Current applied research includes

(1). Use of non-replicating particulate structures for vaccine and immunogen delivery systems. We have begun preparing virus-like particles as vaccines for a number of viruses, such as SARS, Rift Valley Fever virus, Rotavirus and HIV and published some of these results in the peer reviewed journals.

(2). Improvement of the baculovirus protein expression system for synthesis of multiprotein viral and cellular complexes.

(3). Development of a disabled single cycle virus vaccine for Bluetongue virus using a reverse genetics system.

Selected publications

Full publications listing (since 2001)