Wren Lab, Helicobacter pylori
Genetic and molecular characterisation of the gastric pathogen Helicobacter pylori
Principal investigator: Nick Dorrell
Co-Investigator: Brendan Wren, Baraa Hussein, Laura Yates
Funder: MRC
Objectives:
- The objectives of the project are to determine the genetic basis by which H. pylori causes peptic gastric disease. Specific aims are to:
- study key genetic regulatory pathways in relation to stress adaptation using transcriptome analysis
- ascertain the importance of biofilm formation ex vivo genomes of Helicobacter genus
Output from the project:
- Dorrell, N., Martino, M.C., Stabler, R.A., Ward, S.J., Zhang, Z.W., McColm, A.A., Farthing, M.J. & Wren, B.W. (1999). Characterization of Helicobacter pylori PldA, a phospholipase with a role in colonization of the gastric mucosa. Gastroenterology 117: 1098-104
- Foynes, S., Dorrell, N., Ward, S.J., Zhang, Z.W., McColm, A.A., Farthing, M.J. & Wren, B.W. (1999). Functional analysis of the roles of FliQ and FlhB in flagellar expression in Helicobacter pylori. FEMS Microbiol Lett 174: 33-9
- Allan, E., Foynes, S., Dorrell, N., Wren, B.W. & Dorrell, N. (1999). Genetic characterisation of the gastric pathogen Helicobacter pylori. In: Methods in Microbiology: Genetic methods for diverse prokaryotes. M.C.M. Smith & R.E. Sockett (eds). London, Academic Press: pp329-348
- Allan, E., Dorrell, N., Foynes, S., Anyim, M. & Wren, B.W. (2000). Mutational analysis of genes encoding the early flagellar components of Helicobacter pylori: evidence for transcriptional regulation of flagellin A biosynthesis. Journal of Bacteriology 182: 5274-7
- Foynes, S., Dorrell, N., Ward, S.J., Stabler, R.A., McColm, A.A., Rycroft, A.N. & Wren, B.W. (2000). Helicobacter pylori possesses two CheY response regulators and a histidine kinase sensor, CheA, which are essential for chemotaxis and colonization of the gastric mucosa. Infect Immun 68: 2016-23
- Hatzifoti, C., Wren, B.W. & Morrow, W.J. (2000). Helicobacter pylori vaccine strategies--triggering a gut reaction. Immunol Today 21: 615-9
- Martino, M.C., Stabler, R.A., Zhang, Z.W., Farthing, M.J., Wren, B.W. & Dorrell, N. (2001). Helicobacter pylori pore-forming cytolysin orthologue TlyA possesses in vitro hemolytic activity and has a role in colonization of the gastric mucosa. Infect Immun 69: 1697-703
- Allan, E., Clayton, C.L., McLaren, A., Wallace, D.M. & Wren, B.W. (2001). Characterization of the low-pH responses of Helicobacter pylori using genomic DNA arrays. Microbiology 147: 2285-92
- Zhang, Z.W., Dorrell, N., Wren, B.W. & Farthingt, M.J. (2002). Helicobacter pylori adherence to gastric epithelial cells: a role for non-adhesin virulence genes. J Med Microbiol 51: 495-502
- Hatzifoti C., Bajaj-Elliott M., Dorrell N., Anyim M., Prentice M.B., Nye K.E., Wren B.W. & Morrow W.J. (2004) A plasmid immunization construct encoding urease B of Helicobacter pylori induces an antigen-specific antibody response and upregulates the expression of beta-defensins and IL-10 in the stomachs of immunized mice. Vaccine 22(20):2651-2659
- Hatzifoti, C., Roussel, Y., Harris, A., Wren, B.W., Morrow, W.J.W., Bajaj-Elliott, M. (2006) Mucusal immunisation with a urease B DNA vaccine induces innate and cellular immune responses against Helicobacter pylori. Helicobacter 11:113-122
- Loman, N.J., Snyder, L.A., Linton, J.D., Langdon, R., Lawson, A.J., Weinstock, G.W., Wren, B.W., Pallen, M.J. (2009) Genome sequence of the emerging pathogen Helicobacter canadensis. J Bacteriol 191(17):5566-7
- Snyder, L.A.; Loman, N.J.; Linton, J.D.; Langdon, R.R.; Weinstock, G.M.; Wren, B.W.; Pallen, M.J. (2010) Simple sequence repeats in Helicobacter canadensis and their role in phase variable expression and C-terminal sequence switching. BMC Genomics Jan 27; 11:67
- Jervis, A.J.; Langdon, R.; Hitchen, P.; Lawson, A.J.; Wood, A.; Fothergill J.L.; Morris. H.R.; Dell, A.; Wren, B.W.; Linton, D. Characterization of N-Linked Protein Glycosylation in Helicobacter pullorum. J. Bacteriol. (2010) Oct;192(19):5228-36
