Luminex 100
 |  |
The Luminex® 100 System is a laser based system that can be used to develop multi-analyte assays. It allows the simultaneous assay of up to 100 analytes in a single well of a microtiter plate by using dyed antibody coated beads which bind the analytes. A reporter antibody with another dye then quantifies the amount of analyte on the bead.
This system can be used for a number of different applications including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays.
Within the unit it is currently being used to determine cytokine concentrations by fluorescence linked immunosorbent assays (Fluorescence ‘ELISAs’) and to quantitatively measure multiple RNA targets.
The advantages over traditional methods are the low volume of sample required (50μl), the ability to perform multiple analyte detection on one sample, sensitivity and speed.
The Machine runs BioRad’s BioPlex software for acquisition and analysis.
Further Information
A booking system is in operation to allow for equal opportunity of access and to aid cost recovery.
For further information please contact elizabeth.king@lshtm.ac.uk
Internal Users can visit the following Network folder: J:/FACS Users/Luminex
Weblinks
Company | Website (updated Jun2010) |
Luminexcorp | |
Affymetrix | |
BioRad | |
Invitrogen | |
Millipore |
LSHTM is not responsible for the content of external sites.
