Dr Sam Alsford BSc MSc PhD
About Sam Alsford
Trypanosomatid molecular & cellular biology researcher, molecular parasitology lecturer, and PMB Research Degrees Coordinator.
- Sam Alsford's Contacts
- Lab 333 & Office 382
- Keppel Street
- WC1E 7HT
- T: 020 7927 2352 (office) 020 7927 2699 (lab)
I've been fascinated by parasite biology since carrying out two research projects on the surface biology of Schistosome blood flukes during my BSc at Imperial College. After completing an MSc in Molecular Biology of Infectious Diseases at LSHTM, I moved to Prof Keith Gull's lab at the University of Manchester, where my PhD studies focused on gene expression and genome organisation in Trypanosoma brucei (the causative agent of human African trypanosomiasis and nagana in cattle, both devastating diseases).
In 2003, I returned to LSHTM, where I worked in Prof David Horn's laboratory on several aspects of T. brucei molecular and cellular biology, including: epigenetics, and the control of gene expression and antigenic variation; molecular tool development and whole genome analysis; and, anti-trypanosomal drug uptake and intracellular transit.
In June 2012, following the award of a two year LSHTM/Wellcome Trust Institutional Strategic Support Fund Fellowship, I established my own laboratory and research programme at LSHTM (see below), further details of which can be found here.
I deliver lectures on the core Parasitology & Entomology (M3122) and Molecular Biology (M3333) modules in term 1. I organise Advanced Training in Molecular Biology (M3158) in term 2, delivering practical and bioinformatics classes. I also contribute to several other modules, including: Recombinant DNA Techniques (M3131) and Molecular Biology Research Applications & Progress (M3160) in term 2; and, Antimicrobial Chemotherapy (M3169) and Pathogen Genomics (M3460), which both run in term 3. More details can be found here.
I'm a tutor for the Molecular Biology of Infectious Diseases and Medical Parasitology MSc courses, and serve on the course committees and exam boards of both.
I'm also Departmental Research Degrees Coordinator for the Pathogen Molecular Biology department (since 1st September 2014).
Since June 2012, I have been pursuing my own research programme focusing on the interactions between Trypanosoma brucei and its host environment (more details can be found here). This work was initially funded by a two year LSHTM/Wellcome Trust Fellowship (2012-14), and subsequently a three year project grant from the Medical Research Council (2013-2016).
I'm particularly interested in the uptake and intracellular transit of parasite- and host-derived molecules, such as nutrients, drugs and innate immune factors. My research is underpinned by a combination of high-throughput forward genetic screens and reverse genetic approaches, enabling the identification and characterisation of proteins involved in the uptake and intracellular transit of these molecules, as well as the parasite's response to them.
I'm currently developing research projects looking at understanding the mode-of-action of novel anti-T. brucei drugs and the current anti-leishmanials (Leishmania, a related kinetoplastid parasite, shares a lot of biochemical characteristics with T. brucei). Other projects under development include: the role of calcium signalling in density sensing and cell cycle control; and, the regulation of rDNA transcription and its impact on expression of the RNA polymerase I-transcribed VSG - the variant surface glycoprotein is a highly abundant protein on the surface coat, whose switching through antigenic variation enables T. brucei to avoid clearance by the mammalian immune system.
The full range of plasmids for tagged protein expression and RNAi knockdown in T. brucei, developed in conjunction with David Horn, are freely available to the research community - details can be found here.
- Drug discovery and development
- Drug resistance
- Innate immunity
- Cell biology
- Molecular biology
Disease and Health Conditions
- African trypanosomiasis
- Antigenic Variation
- Drug Efficacy
- Gene Expression
- Histone Modifications
VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes.
Glover, L. ; Hutchinson, S. ; Alsford, S. ; Horn, D. ;
Proc Natl Acad Sci U S A, 2016;
Co-dependence between trypanosome nuclear lamina components in nuclear stability and control of gene expression.
Maishman, L. ; Obado, S.O. ; Alsford, S. ; Bart, J.M. ; Chen, W.M. ; Ratushny, A.V. ; Navarro, M. ; Horn, D. ; Aitchison, J.D. ; Chait, B.T. ; Rout, M.P. ; Field, M.C. ;
Nucleic Acids Res, 2016;
Modulation of the Surface Proteome through Multiple Ubiquitylation Pathways in African Trypanosomes.
Zoltner, M. ; Leung, K.F. ; Alsford, S. ; Horn, D. ; Field, M.C. ;
PLoS Pathog, 2015; 11(10):e1005236
Cathepsin-L Can Resist Lysis by Human Serum in Trypanosoma brucei brucei.
Alsford, S. ; Currier, R.B. ; Guerra-Assunção, J.A. ; Clark, T.G. ; Horn, D. ;
PLoS Pathog, 2014; 10(5):e1004130
High-throughput decoding of antitrypanosomal drug efficacy and resistance.
Alsford, S. ; Eckert, S. ; Baker, N. ; Glover, L. ; Sanchez-Flores, A. ; Leung, K.F. ; Turner, D.J. ; Field, M.C. ; Berriman, M. ; Horn, D. ;
Nature, 2012; 482(7384):232-6
Cell-cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei.
Alsford, S.; Horn, D.;
Nucleic Acids Res, 2012; 40(20):10150-60
Elongator Protein 3b Negatively Regulates Ribosomal DNA Transcription in African Trypanosomes.
Alsford, S.; Horn, D.;
Mol Cell Biol, 2011; 31(9):1822-32
High throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome.
Alsford, S.; Turner, D.J.; Obado, S.O.; Sanchez-Flores, A.; Glover, L.; Berriman, M.; Hertz-Fowler, C.; Horn, D.;
Genome Res, 2011; 21(6):915-24
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